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A modified SELEX approach to identify DNA aptamers with binding specificity to the major histocompatibility complex presenting ovalbumin model antigen

10.1001/jama.2016.10260 53 MarsoS

Changes in gene expression were determined by normalizing mRNA levels to those of GAPDH as an internal control and fold change was calculated using the 2 CT method

It has been proposed that also this basement membrane-associated GPX3 is mostly kidney-derived 32 , whereas our results suggest that bronchial epithelial cells secrete GPX3 and thus contribute to the subepithelial basement membrane-associated population