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120 A significant reduction in the rate of CoQ biosynthesis occurs during aging and age-associated diseases

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Malondialdehyde (MDA), used as an index of lipid peroxidation, was determined via the thiobarbituric acid reaction method described by 2 O 2 ) content was measured using the FOX1 assay, which relies on the peroxide-dependent oxidation of Fe 2+ , following 2.5.2 ROS-generating and antioxidant enzymes The activities of NADPH oxidase and the antioxidant enzymes ascorbate peroxidase (APX), superoxide dismutase (SOD), and catalase (CAT) were quantified to assess enzymatic defenses